After the successful launch of its SensoLyte® 520 Thiol Quantitation Assay Kit (patent pending), AnaSpec received several customer inquiries for a sensitive glutathione (GSH) assay. In response, AnaSpec has released the SensoLyte® 520 Total GSH Assay Kit (patent pending).
The SensoLyte® 520 Total GSH Assay Kit provides a convenient and highly sensitive fluorescent assay for the measurement of total GSH (GSH+GSSG). It employs a novel fluorogenic reagent, which upon reaction with reduced GSH releases fluorescence. Fluorescence is monitored at Ex/Em= 490/520 nm. Additionally, oxidized GSH can be converted to the reduced GSH by glutathione reductase and total GSH level can be quantified. The assay has a linear range of 0.2-100 mM of glutathione. The kit also provides sulfosalicylic acid for deproteination of biological samples.
A colorimetric GSH kit, the SensoLyte® Total GSH Assay Kit is also available. This kit contains Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)], which upon reaction with reduced GSH releases a colored product, monitored at 415 nm using a microplate reader. The intensity of the color produced is proportional to GSH concentration.
Reduced Glutathione (GSH) is the main thiol (sulfhydryl) compound in animal, plant and protozoa. GSH plays a crucial role in cellular defense against oxidative stress and functions as a cofactor for a variety of enzymes such as glutathione transferase and glutathione peroxidase. GSH is also involved in the maintenance of redox status, amino acid transport, and mitochondrial respiration.1,2 Total GSH includes the reduced and oxidized form (GSSG). Upon oxidation, GSH is converted to GSSG, with the ratio of GSH/GSSG decreasing due to GSSG accumulation. The concentrations of GSH and GSSG and their molar ratio are indicators of the cell’s functionality and oxidative stress level.3, 4
1. Dickinson, DA. et al. Ann. N. Y. Acad. Sci. 937, 488 (2002).
2. Townsend, DM. et al. Biomed. Oharmacother. 57, 145 (2003).
3. Ballatori, N. et al. Biol. Chem. 390, 191 (2009).
4. Rahman, I et al. Antioxid. Redox Signal. 7, 42 (2005).
AnaSpec (anaspec.com) is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.
AnaSpec recently joined forces with Eurogentec to provide an even broader spectrum of products and services to serve the worldwide life science community.
Eurogentec (eurogentec.com) is a leading global supplier of innovative reagents, kits, specialty products and custom services to scientists in the life science, biotechnology, pharmaceutical and diagnostic markets. Eurogentec provides a wide range of expertize in small- and large-scale DNA, RNA, PCR and qPCR kits, peptide synthesis and antibody supply for research applications. Our ISO13485:2003-certified manufacturing facilities in Belgium provides a wide range of high value oligonucleotide-based components for diagnostic and therapeutic applications. Eurogentec’s Belgium manufacturing facility is complemented by additional production facilities in North America and Japan. Eurogentec is also an experienced Contract Manufacturing Organization (CMO) for Biopharmaceuticals, operating a full-service, state-of-the-art GMP facility in Belgium.
Eurogentec is a privately held company headquartered in Liège, Belgium, with subsidiaries in North America, France, Germany, the UK, the Netherlands and Switzerland and has additional production facilities in North America, Japan and Singapore. Eurogentec employs 400+ people globally.