NewswireTODAY - /newswire/ - Newmarket, Suffolk, United Kingdom, 2014/03/17 - VeriQuest SYBR Green qPCR Master Mix - Stratech.co.uk.

VeriQuest SYBR Green qPCR Master Mix is supplied as a 2X pre-mixed formulation containing both ROX and UDG in an optimized buffer for quality results in real-time quantitative PCR assays. The proprietary reaction buffer and the hot start polymerase enhance SYBR Green-based qPCR amplification by reducing primer-dimer formation and non-template priming, increasing specificity and sensitivity. The master mix contains a chemically-modified Taq DNA Polymerase, MgCl2, ultrapure nucleotides with an optimized dUTP:dTTP ratio, Uracil-DNA Glycosylase (UDG or UNG), SYBR Green I, and ROX Passive Reference Dye in a proprietary reaction buffer.

Features:

• One-tube master mix with ROX reference dye included;
• Same protocols and same primers for standard mode amplification
• Consistency over a broad dynamic range: 7 orders of magnitude linear detection range with a correlation coefficient approximately equal to 1;
• Exceptional performance with challenging GC-rich regions;
• Detect less than 4 copies of target from genomic DNA and differentiate less than a two-fold difference;
• Contains dUTP and Uracil-DNA Glycosylase (UDG or UNG) for carryover contamination prevention;
• Hot start PCR for room temperature reaction setup;
• Stable at room temperature for 72 hours in a preassembled reaction.

Agency / Source: Stratech Scientific Ltd

Availability: All Regions (Including Int'l)

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