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NewswireTODAY - /newswire/ -
Fremont, CA, United States, 2012/10/08 - Leveraging our world-class peptide synthesis expertise and antibody production experience, AnaSpec, EGT Group is pleased to offer high quality custom monoclonal antibody services to the research community and pharmaceutical industry.
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We have the capabilities to raise monoclonal antibodies to difficult antigens, including peptide sequences that contain post-translational modifications.
• Consultation in peptide antigen design;
• Peptide antigens with post-translational modifications can be made;
• Flexible protocol and schedule based on customer’s needs;
• Immunization time frame of four to eight weeks;
• Up-to-date report and customer validation following each project step;
• Accessible technical support with expertise in antibody production;
• Resources and technical proficiency to work with customer on unconventional projects.
Our routine production of monoclonal antibodies is comprised of three phases (Phase I – III). Customers only pay for each phase that is successfully completed. Phase IV, large-scale production of monoclonal antibodies, is optional and available as a stand-alone purchase. Details of each phase are shown below.
Phase I: Antigen Preparation and Immunization (~8-9 weeks);
• Design and production of synthetic peptide antigens with or without modifications;
• Site-directed conjugation of peptide antigens to carrier proteins (e.g., KLH and BSA) for flexibility in antigen presentation;
• Preparation of peptide and protein antigens with adjuvants for immunization;
• Initial and boost immunizations of at least four animals;
• Serum separation, ELISA testing and customer consultation before proceeding to Phase II.
Phase II: Fusion and Selection of Parental Clones (~3 weeks)
• Splenocytes are harvested for cell fusion from mice with the highest titer;
• Clones of fused cells are expanded and screened by ELISA;
• Supernatants, from up to ten ELISA-positive parental clones, are shipped to customer for further antibody testing in immunoassays;
• The best parental clones (up to three) are chosen for Phase III after customer consultation.
Phase III: Subcloning and selection of Single Clones (~3 weeks)
• Parental clones are sub-cloned by limiting dilution to isolate single clones;
• Isotype determination of single clones – IgG subtype guaranteed single clones are expanded and screened by ELISA;
• Single clones with high specificity (e.g., phospho vs. non-phospho) and antibody titer will be expanded and cryopreserved;
• Three hybridomas, and their supernatants, will be shipped to customer.
Phase IV: Large-Scale Production of Monoclonal Antibodies (optional) (~3-6weeks)
• In vivo ascites or in vitro Bioreactor antibody production is available;
• Purification of monoclonal antibodies by affinity chromatography using Protein G or with the immunizing peptide antigen;
• ELISA and SDS-PAGE are done to validate the purity of the antibody;
• AnaSpec offers a range of production scales with flexible cost adjustment.
In addition to our custom services, AnaSpec, EGT Group offers popular catalog monoclonal antibodies such as the anti-amyloid (1-40) and (1-42) antibodies.
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Agency / Source: AnaSpec, Inc.
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Availability: All Regions (Including Int'l)
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Custom Hybridoma
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Contact: Tiffany Kao - AnaSpec.com
510-791-9560
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