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AnaSpec Introduces HTS-Optimized EnzoLyte™ Caspase Assay Kits - Apoptosis, a programmed, cell-autonomous death process, plays important roles in a variety of physiological and pathological events1.
AnaSpec Introduces HTS-Optimized EnzoLyte™ Caspase Assay Kits

 

NewswireToday - /newswire/ - San Jose, CA, United States, 2006/09/12 - Apoptosis, a programmed, cell-autonomous death process, plays important roles in a variety of physiological and pathological events1..

   
 
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These range from normal fetal development to diseases, such as cancer2, organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase3, DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C delta4. At least ten caspases have been discovered. Some of these caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate4.

AnaSpec’s line of EnzoLyte™ Caspase Assay Kits are optimized for high-throughput screening. HTS-optimized features include: one-step homogeneous assays, a convenient mix-and-read format, and highly sensitive fluorimetric components.

EnzoLyte™ Homogeneous Caspase-3/7 Assay Kits
- For detecting caspase 3/7 activity in cells and/or for screening caspase 3/7 inducers or inhibitors
- Measures activity directly in 96- or 384-well microplate, bifunctional lysis buffer lyses cells as well as provides optimal buffer condition for caspase 3/7 activity

EnzoLyte™ Homogeneous AFC Caspase-3/7 Assay Kit
· 500 assays in 96-well format
· Ex/Em(nm)=370/500

EnzoLyte™ Homogeneous AMC Caspase-3/7 Assay Kit
· 500 assays in 96-well format
· Ex/Em(nm)=340/440

EnzoLyte™ Homogeneous Rh110 Caspase-3/7 Assay Kit
· 500 assays in 96-well format
· Ex/Em(nm)=501/527

EnzoLyte™ Caspase Profiling Kits
- For assaying caspase protease activities

EnzoLyte™ AMC Caspase Profiling Kit
· 8 AMC-based substrates coated to two 96-well plates, AMC fluorescence reference std coated on the same plate
· Ex/Em=340/440 nm
EnzoLyte™ AFC Caspase Profiling Kit
· 8 AFC-based substrates coated to two 96-well plates, AFC fluorescence reference std coated on same plate
· Ex/Em=370/500 nm
EnzoLyte™ Caspase Substrate Sampler Kits
- The best solution for identifying a suitable substrate for designing AFC-based caspase assays

EnzoLyte™ AMC Caspase Substrate Sampler Kit
· 8 AMC-based substrates in individual vials. Each substrate contains ample amount for 100 assays in a 96-well format
· Ex/Em=340/440 nm
EnzoLyte™ AFC Caspase Substrate Sampler Kit
· 8 AFC-based substrates in individual vials. Each substrate contains ample amount for 100 assays in a 96-well format
· Ex/Em=370/500 nm

References
1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, YA. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).

 
 
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Written by / Agency / Source: AnaSpec, Inc.

 
 

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AnaSpec Introduces HTS-Optimized EnzoLyte™ Caspase Assay Kits

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Publisher Contact: Debra Thai 
800-452-5530 marketing[.]anaspec.com
 
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