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AnaSpec Introduces the release of Mant-ATP and Mant-GTP - AnaSpec is pleased to announce the release of Mant-ATP and Mant-GTP
AnaSpec Introduces the release of Mant-ATP and Mant-GTP

 

NewswireToday - /newswire/ - Fremont, CA, United States, 2010/08/25 - AnaSpec is pleased to announce the release of Mant-ATP and Mant-GTP.

   
 
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Mant-ATP and Mant-GTP are the fluorescent analogs of ATP and GTP where either the ribose 2’-hydroxy or the 3’-hydroxy group is esterified by the fluorescent methylisatoic acid (Figure 1). The fluorescence quantum yield of Mant fluorophore is 0.2 in water, which increases significantly in nonpolar solvents or upon binding to most proteins. The highly environmentally sensitive fluorescence of Mant makes Mant-ATP and Mant-GTP useful probes for directly detecting nucleotide-protein interactions at Ex/Em=355/448 nm.

With >95% purity, AnaSpec’s Mant-ATP and Mant-GTP offer cost-effective value without compromising performance.

References:

1. Mocz, G. et al. Probing the Nucleotide Binding Sites of Axonemal Dynein with the Fluorescent Nucleotide Analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate. Biochemistry, 1998, 37, 9862-9869.

2. Bauer, C.B. et al. X-ray Crystal Structure and Solution Fluorescence Characterization of Mg-2'(3')-(N-Methylanthraniloyl) Nucleotides Bound to the Dictyostelium discoideum Myosin Motor Domain. J. Mol. Biol., 1997, 274, 394-407.

3. Hiratsuka, T., New Ribose-modified Fluorescent Analogs of Adenine and Guanine Nucleotides Available as Substrates for Various Enzymes. Biochim. Biophys. Acta, 1983, 742, 496-508.

4. Cunliffe, J.M. et al. Detection of Adenylyl Cyclase Activity Using a Fluorescent ATP Substrate and Capillary Electrophoresis. Anal. Chem., 2006, 78, 1731-1738.

5. Mou, T.-C. et al. Structural Basis for the Inhibition of Mammalian Membrane Adenylyl Cyclase by 2'(3')-O-(N-Methylanthraniloyl)-guanosine 5'-triphosphate. J. Biol. Chem., 2005, 280, 7253-7261.

6. Remmers, A. E. et al. Fluorescent Guanine Nucleotide Analogs and G Protein Activation. J. Biol. Chem., 1994, 269, 13771-13778

7. Hazlett, T.L. et al. Solution Dynamics of p21ras Proteins Bound with Fluorescent Nucleotides: a Time-resolved Fluorescence Study. Biochemistry, 1993, 32, 13575-13583.

8. Eccleston, J.F. et al. Fluorescence Approaches to the Study of the p21ras GTPase Mechanism. Biochem. Soc. Trans., 1991, 19, 432-437.

About AnaSpec

AnaSpec (anaspec.com) is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.

AnaSpec recently joined forces with Eurogentec to provide an even broader spectrum of products and services to serve the worldwide life science community.

About Eurogentec
Eurogentec (eurogentec.com) is a leading global supplier of innovative reagents, kits, specialty products and custom services to scientists in the life science, biotechnology, pharmaceutical and diagnostic markets. Eurogentec provides a wide range of expertize in small- and large-scale DNA, RNA, PCR and qPCR kits, peptide synthesis and antibody supply for research applications. Our ISO13485:2003-certified manufacturing facilities in Belgium provides a wide range of high value oligonucleotide-based components for diagnostic and therapeutic applications. Eurogentec’s Belgium manufacturing facility is complemented by additional production facilities in North America and Japan. Eurogentec is also an experienced Contract Manufacturing Organization (CMO) for Biopharmaceuticals, operating a full-service, state-of-the-art GMP facility in Belgium.

Eurogentec is a privately held company headquartered in Liège, Belgium, with subsidiaries in North America, France, Germany, the UK, the Netherlands and Switzerland and has additional production facilities in North America, Japan and Singapore. Eurogentec employs 400+ people globally.

 
 
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AnaSpec Introduces the release of Mant-ATP and Mant-GTP

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Publisher Contact: Tiffany Kao - AnaSpec.com 
510-791-9560
 
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