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Fremont, CA, United States, 2010/05/26 - AnaSpec has introduced two new Glutathione (GSH) related kits - the SensoLyte® Glutathione Cellular Assay Kit and the SensoLyte® GST Activity Assay Kit to complement the colorimetric and fluorimetric SensoLyte® Total GSH assay kits.
The SensoLyte® Glutathione Cellular Assay Kit provides a sensitive fluorescent assay for determination of the glutathione levels in cell or tissue extracts. The kit contains a non-fluorescent substrate, which selectively reacts with GSH in reaction catalyzed by glutathione S-transferase (GST) to form a fluorescent glutathione adduct. The resulting blue fluorescence can be monitored at Ex/Em= 390 nm/480 nm.
The SensoLyte® GST Activity Assay Kit detects total GST activity in plasma, cell and tissue lysates. It contains a non-fluorescent substrate for GST, which upon conjugation with reduced GSH generates bright fluorescence that can be detected at Ex/Em= 390nm/480 nm.
Glutathione S-transferases (GST) belong to a family of enzymes that is involved in detoxification and toxification mechanisms. They act via conjugation of reduced glutathione (GSH) with numerous substrates such as pharmaceuticals and environmental pollutants.1-4 GST enzymes are believed to be crucial to the body's defense against cancer-causing chemicals and other toxins. These enzymes also carry out a wide range of functions in cells such as removal of reactive oxygen species (ROS) and regeneration of S-thiolated proteins, both of which are consequences of oxidative stress.
Figure 1. The substrate found in the SensoLyte® GST Activity Assay kit (Cat# 72157) catalyzes an enzymatic conversion of non-fluorescent monochlorobimane to a fluorescent glutathione adduct by glutathione S-transferase.4
1. Sheenan D. et al. Biochem J. 360, 1 (2001).
2. Rushmore T. et al. J. Biol. Chem. 268, 11475 (1993).
3. Estonius M. et al. Eur. J. Biochem. 260, 409 (1999).
4. Hayes D. J et al. Annu. Rev. Pharmacol. Toxicol. 45, 51 (2005).
AnaSpec (anaspec.com) is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.
AnaSpec recently joined forces with Eurogentec to provide an even broader spectrum of products and services to serve the worldwide life science community.
Eurogentec (eurogentec.com) is a leading global supplier of innovative reagents, kits, specialty products and custom services to scientists in the life science, biotechnology, pharmaceutical and diagnostic markets. Eurogentec provides a wide range of expertize in small- and large-scale DNA, RNA, PCR and qPCR kits, peptide synthesis and antibody supply for research applications. Our ISO13485:2003-certified manufacturing facilities in Belgium provides a wide range of high value oligonucleotide-based components for diagnostic and therapeutic applications. Eurogentec’s Belgium manufacturing facility is complemented by additional production facilities in North America and Japan. Eurogentec is also an experienced Contract Manufacturing Organization (CMO) for Biopharmaceuticals, operating a full-service, state-of-the-art GMP facility in Belgium.
Eurogentec is a privately held company headquartered in Liège, Belgium, with subsidiaries in North America, France, Germany, the UK, the Netherlands and Switzerland and has additional production facilities in North America, Japan and Singapore. Eurogentec employs 400+ people globally.