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New SensoLyte® SIRT1 Assay Kit Offers Industry’s Longest Wavelength - AnaSpec has introduced SIRT1 assay kits in the green range (Ex/Em=490/520 nm)
New SensoLyte® SIRT1 Assay Kit Offers Industry’s Longest Wavelength

 

NewswireToday - /newswire/ - Fremont, CA, United States, 2010/04/30 - AnaSpec has introduced SIRT1 assay kits in the green range (Ex/Em=490/520 nm).

   
 
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AnaSpec, Eurogentec’s newest North American division, has introduced SIRT1 assay kits in the green range (Ex/Em=490/520 nm). These kits, the SensoLyte® 520 FRET SIRT1 Assay Kit and SensoLyte® Green SIRT1 Assay Kit, represent the industry’ first and longest wavelength SIRT 1 assays. These two-step, homogeneous assays use a FRET substrate with 5-FAM/QXL™ 520 as the FRET pair in the former and a green fluorophore containing substrate in the latter. The long wavelength fluorescence of the green dyes employed in these two kits is less interfered by the autofluorescence of cell components and test compounds; thus making these kits ultra-sensitive.

Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone.1 Sirtuins comprise a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases (class III HDACs) that target multiple protein substrates to execute diverse biological functions. Sirtuins catalyze a reaction that couples lysine deacetylation to NAD hydrolysis, yielding O-acetyl-ADP-ribose and nicotinamide.2

Sirtuin 1 (SIRT1), the human homolog of yeast Sir2 (Silent Information Regulator 2), is the most studied of the seven members of sirtuin family. SIRT1 have been implicated in several important cellular processes, including genomic stability and DNA repair,3,4 p53-mediated apoptosis,5 adipogenesis,6 and aging.7, 8

References:
1. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).
2. Longo, V and Kennedy, B. Cell. 126, 257 (2006).
3. Yamagata, K and Kitabayashi, I. Biochem Biophys Res Commun. 390, 1355 (2009).
4. Wang, RH. et al. Cancer Cell. 14, 312 (2008).
5. Vaziri, H. et al. Cell. 107, 149 (2001).
6. Picard, F. et al. Nature. 429, 771 (2004).
7. Cohen, HY. et al. Science 305, 390 (2004).
8. Trapp, J. and Jung, M. Curr. Drug Target 7, 1553 (2006).

About AnaSpec
AnaSpec (anaspec.com) is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.

AnaSpec recently joined forces with Eurogentec to provide an even broader spectrum of products and services to serve the worldwide life science community.

About Eurogentec
Eurogentec (eurogentec.com) is a leading global supplier of innovative reagents, kits, specialty products and custom services to scientists in the life science, biotechnology, pharmaceutical and diagnostic markets. Eurogentec provides a wide range of expertize in small- and large-scale DNA, RNA, PCR and qPCR kits, peptide synthesis and antibody supply for research applications. Our ISO13485:2003-certified manufacturing facilities in Belgium provides a wide range of high value oligonucleotide-based components for diagnostic and therapeutic applications. Eurogentec’s Belgium manufacturing facility is complemented by additional production facilities in North America and Japan. Eurogentec is also an experienced Contract Manufacturing Organization (CMO) for Biopharmaceuticals, operating a full-service, state-of-the-art GMP facility in Belgium.

Eurogentec is a privately held company headquartered in Liège, Belgium, with subsidiaries in North America, France, Germany, the UK, the Netherlands and Switzerland and has additional production facilities in North America, Japan and Singapore. Eurogentec employs 400+ people globally.

 
 
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Written by / Agency / Source: AnaSpec, Inc.

 
 

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New SensoLyte® SIRT1 Assay Kit Offers Industry’s Longest Wavelength

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Publisher Contact: Ping Yang - AnaSpec.com 
510-791-9560 ping[.]anaspec.com
 
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