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AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate - AnaSpec, Inc. is a leading provider of integrated proteomics solutions for worldwide life science research.
AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate

 

NewswireToday - /newswire/ - San Jose, CA, United States, 2006/07/12 - AnaSpec, Inc. is a leading provider of integrated proteomics solutions for worldwide life science research..

   
 
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With a vision for innovation through synergy, AnaSpec offers expertise in three primary technologies: peptides, detection reagents, and combinatorial chemistry. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. In conjunction, AnaSpec provides premier custom services including peptide synthesis, antibody production, and assay development. AnaSpec holds a California Drug Manufacturing License (License #41747) and a FDA Registration for GMP Drug Manufacturing (Registration #2951078).

NS3/4A protease is the protease responsible for the cleavage of HCV nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites. Since these cleavages are essential for the maturation of the viral proteins, this protease has become one of the key targets for developing anti-HCV drugs. Focusing on this particular protease, AnaSpec has developed an innovative new tool for optimized detection performance.

Applying both peptide and fluorescent dye technologies, AnaSpec has developed an ultra-sensitive HCV NS3/4A protease FRET substrate that can detect the presence of HCV NS3/4A protease at levels of < 0.1 pmol. Based on the peptide sequence, Ac-DED(EDANS)EE-Abu-[y,COO]ASK(DABCYL)-NH2, developed by Taliani, M. et al. (1), the donor and the quencher have been substituted with 5-FAM and our trademark quencher QXLTM 520, respectively. This substrate, Ac-DE-DAP(QXL520)-EE-Abu-[y,COO]AS-C(5-FAMsp)-NH2, shows a 22-fold lower Km than the EDANS/DABCYL peptide.

References:

1. Taliani, M. et al., Anal.Biochem. 240, 60-67 (1996).

 
 
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AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate

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Publisher Contact: Debra Thai 
408-452-5055 marketing[.]anaspec.com
 
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