Thermo Fisher Scientific, Inc., the world leader in serving science, today announced a new application note that illustrates the superior capabilities of dual-pressure linear ion trap technology for the investigation of proteome dynamics. The application note assessed the performance of the newly launched Thermo Scientific LTQ Velos ion trap mass spectrometer for the analysis of a complex, multi-organ peptide digest of Caenorhabditis elegans (C. elegans). The LTQ Velos™ ion trap mass spectrometer offers improvements in speed and sensitivity, providing greater coverage in the analysis of complex peptide mixtures and increasing the confidence of protein identification at low sample levels.
Tandem mass spectrometry, particularly using linear ion traps and trap-based hybrid mass spectrometers, has become a leading technology for peptide and protein identification. The preference for this technology is due to its ease of use as well as its superior MS and MS/MS performance. The multiple fragmentation techniques available on the LTQ Velos enable more confident sequence assignment and post-translational modification (PTM) identification. Faster scan rates also significantly reduce cycle times, increasing the number of proteins and peptides identified.
The application note describes an effective method for the analysis of peptides and proteins using the LTQ Velos mass spectrometer. To compare and benchmark performance, the company ran analyses on both a Thermo Scientific LTQ XL linear ion trap mass spectrometer and a quadrupole time-of-flight (Q-TOF) mass spectrometer. The study used data-dependent tandem acquisition methods, and the team separated a proteolytic digest of C. elegans using reverse phase chromatography for each LC-MS/MS run. The analysis revealed that the LTQ Velos identifies ~240 percent more proteins and ~130 percent more distinct peptides from a highly complex sample than is possible with the Q-TOF.
For more information about the Thermo Scientific LTQ Velos or to obtain a copy of the application note, please call 1-800-532-4752 or E: analyze[.]thermo.com.
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About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (thermofisher.com) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With 2008 revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike.