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Thermo Fisher Scientific UHPLC Enables Enhanced Resolution Separations of Complex Drug Mixtures - Thermo Fisher Scientific Inc., the world leader in serving science, has issued a new application note to demonstrate superior ultra high performance liquid chromatography (UHPLC) capabilities for the separation of drugs of abuse in complex drug mixtures
Thermo Fisher Scientific UHPLC Enables Enhanced Resolution Separations of Complex Drug Mixtures

 

NewswireToday - /newswire/ - San Jose, CA, United States, 2009/07/31 - Thermo Fisher Scientific Inc., the world leader in serving science, has issued a new application note to demonstrate superior ultra high performance liquid chromatography (UHPLC) capabilities for the separation of drugs of abuse in complex drug mixtures.

   
 
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Illustrated in the application note is the advanced ability of the Thermo Scientific Accela UHPLC system to perform efficient, improved resolution separations using a unique quaternary UHP mixing pump. The new application note, entitled "A Comparison of Binary and Ternary Gradients Using Stage 1 Illicit Drugs," is available for download.

When using an HPLC method to separate a complex drug mixture, a simple binary solvent gradient can often fail to provide sufficient resolution of all analytes. In order to improve the resolution, the separation factor has to be manipulated by using a third solvent. However, this approach is not possible with typical high-pressure binary solvent mixing equipment. The Accela UHPLC system overcomes this limitation, featuring a newly introduced quaternary mixing pump that facilitates the use of up to four solvents for convenient method development, system flushing and eluent preparation.
Changing of solvents, buffers and pH values is quick and easy, greatly simplifying the method development process.

The new application note describes an effective method for the separation of drugs using the Accela UHPLC in conjunction with the Thermo Scientific MSQ Plus Mass Detector, a fast-scanning single quadrupole mass spectrometer (MS). A mixture of 14 drugs is separated and baseline resolved by UHPLC/MS within eight minutes on a Thermo Scientific Hypersil GOLD PFP column using a ternary solvent gradient, which is not possible using a conventional binary solvent gradient. Methanol was found to provide improved resolution for most of the drug compounds compared with other eluents.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With 2008 revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike.

For further press information please contact: Celine Callender, The Scott Partnership, 1 Whiteside, Station Road, Holmes Chapel, Cheshire. CW4 8AA United Kingdom T: +44 1477 539539 F: +44 1477 539540 E: thermo[.]scottpr.com.

 
 
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Thermo Fisher Scientific UHPLC Enables Enhanced Resolution Separations of Complex Drug Mixtures

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Publisher Contact: Celine Callender 
+1 866-463-6522 analyze[.]thermofisher.com
 
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