Zebrafish (Danio rerio) as an experimental model has gained widespread use since the pioneering days of Dr. George Streisinger. In the year 1980, there were 18 publications, 72 in 1990, 540 in 2000 and 1159 in 2008.1 To date, there are more than 13,200 publications.2 In order to address the growing needs of the research community for zebrafish specific antibodies, peptides and other products, AnaSpec has announced the launch of the Z-Fish™ product line of antibodies.
Z-Fish™ antibodies are raised strictly from peptide antigens derived from zebrafish protein sequences, i.e. 100% zebrafish sequence homology. Leveraging AnaSpec’s expertise in peptide technology, the antigens used in raising these antibodies include non-phosphorylated and phosphorylated peptides. The antibodies are purified using proprietary purification methods.
The list of Z-Fish™ antibodies includes targets used in apoptosis and autophagy, cell cycle control, cytoskeleton, glucose and energy metabolism, signal transduction, and immunity research. Antibodies were verified in ELISA and in western blots, using zebrafish lysates. Z-Fish™ antibodies are supplied in 150 ul sizes and are enough for 15 mini gels.
Z-Fish™ cytoskeleton antibodies include anti-actin, a-smooth muscle, anti-b-actin, raised with peptide antigens either from the C or N-terminus of the protein, anti-keratin, and b-Catenin, an actin related product. b-Catenin, a protein that bridges between cadherins and actin and plays a central role in cell adhesion, can be phosphorylated by GSK-3.3-6 One phosphorylated and two non-phosphorylated antibodies are available for this protein.
1. ZFIN; Retrieved Apr. 15, 2009.
2. Highwire; Retrieved Apr. 15, 2009.
3. Dale, TC. Biochem. J. 329, 209 (1998).
4. Sadot, E. et al. J. Cell Sci. 115, 2771 (2002).
5. Giannini, A. et al. J. Biol. Chem. 275, 21883 (2000).
6. Thilo, H. et al. J. Biol. Chem. 277, 2330 (2002).
AnaSpec (anaspec.com) is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and combinatorial chemistry.