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Arlington, VA, United States, 2008/03/04 - When exposed to oxidative radicals, 8-hydroxy-2’-deoxyguanosine (8-oxo-dG) is formed and can serve as a sensitive indicator of physiological and environmental damage to DNA.
DNA damage caused by oxidative radicals is associated with aging, inflammation, carcinogenesis, Parkinson’s and Alzheimer’s diseases. When exposed to oxidative radicals, 8-hydroxy-2’-deoxyguanosine (8-oxo-dG) is formed and can serve as a sensitive indicator of physiological and environmental damage to DNA. This mouse monoclonal antibody specifically binds to 8-hydroxy-2’-deoxyguanosine within DNA in H2O2-treated cells.
It can be used to detect oxidative damage by ELISA and immunocytochemistry. For optimal outcomes, cells should be grown on a surface that allows for fixation and direct labeling such as sterile chamber slides and coverslips. This antibody is provided as purified immunoglobulin from mouse ascites in 1X PBS containing 0.01% sodium azide.
Sufficient antibody is provided for approximately 50 slides, when a 1:250 dilution is used (Cat# 4354-MC-050). For details, contact Trevigen by phone at 800-873-8443, by email, or visit the website.
Trevigen (trevigen.com) is a long standing provider of quality kits and reagents for researchers investigating programmed cell death, DNA damage and repair, cell differentiation and angiogenesis. Trevigen recently launched Anti-8-oxo-dG Monoclonal Antibody (Clone 2E2) as part of it DNA Damage product line.
1 Soultanakis RP, Melamede RJ, Bespalov IA, Wallace SS, Beckman KB, Ames BN, Taatjes DJ, Janssen-Heininger YMW. (2000) Flourescence detection of 8-oxoguanine in nuclear and mitochondrial DNA of cultured cells using a recombinant Fab and confocal scanning laser microscopy. Free Rad Biol Med 28:987-998.