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Trevigen Launches High-Throughput PARP/Apoptosis Assays - Trevigen launches High-Throughput PARP/Apoptosis Assays ideal for measuring the activity of PARP in 96-well plates
Trevigen Launches High-Throughput PARP/Apoptosis Assays

 

NewswireToday - /newswire/ - Arlington, VA, United States, 2008/02/22 - Trevigen launches High-Throughput PARP/Apoptosis Assays ideal for measuring the activity of PARP in 96-well plates.

   
 
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Cell extracts can be obtained using our newly-developed, higher-throughput procedure before, during and after apoptosis induction.

During apoptosis, PARP-1 which catalyzes the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from about 116 kDa to 85 kDa. Trevigen's HT PARP/Apoptosis Assay is ideal for measuring the activity of PARP in cell extracts before and during apoptosis. The HT PARP/Apoptosis Assay is an ELISA which semi-quantitatively detects PAR deposited onto immobilized histone proteins in a 96-well format. An anti-PAR monoclonal antibody, goat anti-mouse IgG-HRP conjugate, and HRP substrate are used to generate a colorimetric signal. Thus, absorbance correlates with PARP activity. Etoposide is a topoisomerase II inhibitor that stabilizes this enzyme after it cleaves DNA. It is included as a control apoptosis inducer.

Trevigen offers two formats of the HT PARP/Apoptosis Assay: Cat#4684-096-K (Colorimetric) and Cat# 4685-096-K (Chemiluminescent). Both assays feature non-radioactive formats and high-throughput 96 test size, as well as sensitivity down to 0.1 mUnits of PARP -- equivalent to less than 500 cells/well. Additional histone-coated strip wells (Cat# 4677-096-P) are available separately. For more details about this product, contact Trevigen by phone at 800-873-8443, email or visit the website.

Trevigen, Inc.
8405 Helgerman Ct.
Gaithersburg, MD 20877
P: 1-800-873-8443 / F: 1-301-560-4973

 
 
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Written by / Agency / Source: Trevigen, Inc.

 
 

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Trevigen Launches High-Throughput PARP/Apoptosis Assays

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HT PARP Assay |
Publisher Contact: Belinda De Leon - Trevigen.com 
1-800-TREVIGEN info[.]trevigen.com
 
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