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Written by / Agency / Source: AnaSpec, Inc.

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AnaSpec Introduces New Line of HABA-Biotin Quantitation Kits - AnaSpec has introduced a new line of HABA Biotin Quantitation Kits. They provide a convenient method for estimating the molar ratio of biotin to protein on biotinylated conjugates and also be used to quantitate the biotin concentration in a solution
AnaSpec Introduces New Line of HABA-Biotin Quantitation Kits

 

NewswireToday - /newswire/ - San Jose, CA, United States, 2008/01/22 - AnaSpec has introduced a new line of HABA Biotin Quantitation Kits. They provide a convenient method for estimating the molar ratio of biotin to protein on biotinylated conjugates and also be used to quantitate the biotin concentration in a solution.

   
 
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Available in two sizes: 120 Assays 72096-120 (120 assays in a 96-well format or 25 assays in a cuvette format)
500 Assays 72096-500 (500 assays in a 96-well format or 100 assays in a cuvette format)

Biotin and avidin (or streptavidin) bind non-covalently with a higher binding affinity than most antigen-antibody interactions. This very tight binding makes labeling proteins with biotin a useful tool for applications such as affinity chromatography and immunoanalytical assays.

The assay utilizes the observation that HABA (4¡¦-hydroxyazobenzene-2-carboxylic acid) shows dramatic spectral changes when it binds to avidin. Free HABA has an absorption peak at 348 nm, while the HABA/avidin complex has strong absorption at 500 nm. Since the affinity between HABA and avidin is relatively weak (Kd=5.8 x 10-6 M) compared to the affinity between biotin and avidin (Kd=1 x 10-15 M), biotin can easily replace HABA from the HABA/avidin complex, resulting in a decrease of absorption at 500 nm. Avidin and HABA at an optimal ratio are included in the assay kit. The kit has a linear range of 2 to 16 ƒÝM.

About AnaSpec, Inc.

AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry.

 
 
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Written by / Agency / Source: AnaSpec, Inc.

 
 

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AnaSpec Introduces New Line of HABA-Biotin Quantitation Kits

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Publisher Contact: Ping Yang 
1-408-452-5055 ping[.]anaspec.com
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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