Basement Membrane Extract can be used for promotion and maintenance of a differentiated phenotype in a variety of cell cultures including primary epithelial cells, endothelial cells, and smooth muscle cells. It has been employed in angiogenesis assays, tumor cell invasion assays, and as a vehicle to augment the tumorigenicity of injected tumor cells in nude mice.
The PathClear" designation means that in addition to standard sterility, endotoxin and MAP testing, the BME is tested by PCR and is clear of a battery of 31 pathogens, including LDEV. Each lot is rigorously qualified in biological performance assays. Available in reduced growth factor form, with and without phenol red, PathClear" BME is expected to be of special interest for in vivo murine research work and other work requiring BME free from viruses, bacteria and mycoplasma.
The many applications for Cultrex Basement Membrane Extract require different thicknesses and concentrations. Examples include use of diluted extract (<5 mg/ml) for support of primary cell propagation and higher concentration (>5 mg/ml) for cell propagation and differentiation studies. Basement Membrane Extract is ideal for Tube Assay, Ring Assay, cell invasion studies, in vivo and in vitro angiogenesis assays, tumorgenicity and 3D Culture Assays.
About Trevigen, Inc.
Trevigen, Inc. develops, manufactures and markets an extensive line of Cultrex® brand products for the promotion of cell growth, cellular differentiation and for the study of cancer cell behavior.