NewswireToday - /newswire/ -
San Jose, CA, United States, 2007/06/20 - Leveraging one of the world’s largest collections of amyloid peptides, AnaSpec has introduced its latest development in novel solutions for amyloid-related research - the new SensoLyte™ 520 Beta-Secretase Assay Kit.
Beta-secretase catalyzes a key step in the production of beta-amyloid peptides seen accumulated in senile plagues of Alzheimer’s disease (AD) brains. In order to facilitate high throughput screening of AD drug candidates, we have developed a new SensoLyte™ 520 beta-secretase assay kit using a fluorescence resonance energy transfer (FRET) peptide, HiLyte Fluor™ 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH (see Figure 1). The sequence of this FRET peptide is derived from the beta-secretase cleavage site of beta-amyloid precursor protein(APP) with Swedish mutation.6 This mutation enhances the susceptibility of APP to beta-secretase and results in an early onset of AD.
This assay has good sensitivity (0.03 mU/ml) with a signal-to-background ratio >10 after a 30-minute incubation. This homogeneous assay can be used to continuously monitor product formation. Validated with known inhibitors, the IC50 value for one of the inhibitors determined with SensoLyte™ 520 beta-secretase assay kit was consistent with published data.
About AnaSpec, Inc.
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry. Established in 1993, AnaSpec's ISO9001:2000 certified headquarters and manufacturing facilities are located in San Jose, CA.
1. Selkoe DJ. Nature 399: A23 (1999)
2. Suzuki, N., et al. Science 264, 1336 (1994)
3. Iwatsubo, T. et al. Neuron 13, 45 (1994)
4. Qi-Takahara, Y. et al. J. Neurosci. 25, 436 (2005)
5. Zhao, G. et al. J. Biol. Chem. 280, 37689 (2005)
6. Mullan, M. et al. Nat.Genet. 1, 345 (1992)