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Fremont, CA, United States, 2012/08/06 - Cellular phosphorylation is a reversible, covalent modification of a protein or lipid that results in the modification of the activity of the phosphorylated molecule by inducing small conformational changes within the molecule.1-2 - AnaSpec.com.
Catalyzed by protein kinases, phosphate groups can be added via the transfer of the terminal phosphate from a phosphate donor, e.g. ATP to an amino acid residue, such as serine, threonine or tyrosine (Figure 1). The functions of phosphorylation, the most prevalent of post-translational modifications, include the alteration of the activity of a substrate, its subcellular localization, binding properties with other proteins.1-2 To better understand phosphorylation and its functional role, the generation and use of phosphospecific antibodies is crucial.
For raising antibodies against phosphopeptides there are 3 critical factors for success: (1) the stability of the phosphorylation, (2) the purity of the peptide and (3) post-development purification of the phosphospecific antibody. With documented experience in meeting all three factors, AnaSpec, EGT Group is a proven source for world-class phosphospecific antibody production.
With full manufacturing facilities located in Fremont, CA, AnaSpec (anaspec.com) is able to maintain ISO9001:2008 manufacturing processes and robust quality validation. Our peptide chemists routinely synthesize sequences containing single and multiple phosphoamino acids using technologies that are fine-tuned to produce high-yield, ultra-pure phosphopeptides. Antibodies generated are then substantiated to be phosphospecific.
AnaSpec offers both polyclonal and monoclonal antibody production services for the generation of phospho and non-phosphospecific antibodies. For polyclonal antibodies, researchers have a choice between two immunization protocols - the Speedy 28-day protocol (an example of results is shown in Figure 2) and the 70-day standard protocol. As a trusted provider to the global research community, we are experienced in fulfilling the most stringent demands for optimizing project success. As well, AnaSpec carries a wide selection of phosphospecific catalog antibodies such as the Anti-p53’s and Anti-TAU’s. Phosphospecific antibodies that are zebrafish reactive are also available.
1. Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; 2002. Section 10.4, Covalent Modification Is a Means of Regulating Enzyme Activity.
2. Cohen, P. Proc R Soc Lond 234, 115 (1998). doi: 10.1098/rspb.1988.0040.