PRTODAY / NewswireToday Free press release distribution service network

Written by / Agency / Source: AnaSpec, Inc.

Check Ads Availability|e-mail Article


Are you the owner of this article?, Turn it PREMIUM with your LOGO instead - and make it 3rd party Ads-Free! within the next hour!

AnaSpec Highlights its Proprietary HiLyte Fluor™ Dye Collection - AnaSpec, EGT Group’s proprietary HiLyte Fluor™ dyes are a series of excellent fluorescent labeling dyes with fluorescence emissions that span the full visible and near infrared spectrum - AnaSpec.com / Eurogentec.com
AnaSpec Highlights its Proprietary HiLyte Fluor™ Dye Collection

 

NewswireToday - /newswire/ - Fremont, CA, United States, 2012/01/17 - AnaSpec, EGT Group’s proprietary HiLyte Fluor™ dyes are a series of excellent fluorescent labeling dyes with fluorescence emissions that span the full visible and near infrared spectrum - AnaSpec.com / Eurogentec.com.

   
 
Your Banner Ad Here instead - Showing along with ALL Articles covering Pharma/BioTech/Nutrition Announcements

Replace these Affiliate Programs at ANYTIME! Your banner here within the next hour. Learn How!


 

The hydrophilic property of most of the HiLyte Fluor™ dyes makes conjugations to protein easier, minimizing the use of organic solvents. The resulting conjugates are relatively resistant to aggregation and precipitation during storage. HiLyte Fluor™ dyes also have better labeling performance than the classic fluorescent labeling dyes such as FITC and rhodamines.

AnaSpec HiLyte Fluor™ dyes is available in a variety of reactive forms. HiLyte Fluor™ conjugates exhibit more intense fluorescence than other spectrally similar conjugates of classic fluorescent dyes such as FITC, TAMRA and ROX under similar excitations. They are more photostable than the classic fluorescent dyes such as FITC, Cy3™ and Cy5™. Also, absorption spectra of HiLyte Fluor™ dyes match the principal output wavelengths of common excitation sources such as 488 nm, 555 nm, 633 nm and 647 nm. They are highly fluorescent over a broad pH range with little pH sensitivity.

Well-separated spectra, HiLyte Fluor™ dyes are enabling many options for multicolor detection of multiple parameters simultaneously in flow cytometry analysis. HiLyte Fluor™ dye-antibody conjugates are validated for Western blot, Immunofluorescence staining and flow cytometry. HiLyte Fluor™ dyes and AnaSpec’s proprietary quenchers QXL™ have been used as fluorophore and quencher pairs for fluorescence “resonance energy transfer (FRET). Can be used to make tandem conjugates with fluorescent proteins, such as PE, APC.
HiLyte Fluor™ 405, available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives, allows for easy conjugation to biopolymers. HiLyte Fluor™ 405 has spectral characteristics similar to those of Alexa Fluor™ 405 (Invitrogen) and DyLight Fluor™ 405 (Pierce) dyes with excitation and emission wavelength at 404/428 nm in PBS buffer (pH 7.4). Conjugates of HiLyte Fluor™ 405 are well excited by the 407 nm spectral line of the Krypton laser and the 408 nm line of the violet laser diode. HiLyte Fluor™ 405 conjugates, whether conjugated to primary tag, secondary antibodies or streptavidin, are prepared in an optimized fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised biological function. Fluorescence signal can be observed at Ex/Em = 404/428 nm.

FITC, the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, has its limitations, such as severe photobleaching in microscopy imaging and pH sensitivity. However, protein conjugates prepared with HiLyte Fluor™ 488 dyes (Ex/Em=497 nm/525 nm) are far more superior to conjugates of FITC (Figure 2). HiLyte Fluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Extinction coefficient is 70,000 M-1cm-1, with a quantum yield of 0.91. Additionally, the fluorescence of HiLyte Fluor™ 488 is not affected by pH (working pH range is 4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9.
HiLyte Fluor™ 555 conjugates are more photostable and brighter than conjugates of Cy3™, the preferred dye for preparing orange fluorescent bioconjugates. Compared to the spectra of Cy3™ dyes, the spectra of HiLyte Fluor™ 555 conjugates are slightly red-shifted, resulting in an optimal match of filters designed for Cy3™ dyes. Extinction coefficient is 150,000 M-1cm-1, with a quantum yield of 0.10. The photostability of HiLyte Fluor™ 555 provides researchers with additional time for image capture.

HiLyte Fluor™ 594 has spectral characteristics similar to those of Texas Red®, with excitation and emission wavelength at 593/615 nm in PBS buffer (pH 7.4), and 596/617 nm when conjugated to macromolecules. The labeling performance and stability properties of HiLyte Fluor™ 594 are better than those of Texas Red®. The higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) of HiLyte Fluor™ 594 are better than those of Alexa Fluor™ 594 (Invitrogen). The fluorescence quantum yield of HiLyte Fluor™ 594 is 0.9 in aqueous solution. HiLyte Fluor™ 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells. Biomolecules conjugated to HiLyte Fluor™ 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.
The spectra of HiLyte Fluor™ 647 conjugates are only slightly red-shifted compared to Cy5™, the preferred dye for preparing near-infrared fluorescent bioconjugates. This slight change in absorption spectrum makes HiLyte Fluor™ 647 dye an optimal match of filters designed for Cy5™ dyes. In a side-by-side comparison of antibody conjugates of HiLyte Fluor™ 647 dyes and Cy5™ conjugates (supplied by other companies), the total fluorescence of HiLyte Fluor™ 647 labeled secondary antibodies is significantly higher than that of Cy5™ conjugates. Extinction coefficient is 250,000 M-1cm-1, with a quantum yield of 0.33. Unlike Cy5™ dyes, HiLyte Fluor™ 647 dyes have very little change in absorption or fluorescence spectra when conjugated to most proteins, oligonucleotides and nucleic acids, thus yielding greater total fluorescence at the same degree of substitution.
With a peak excitation at 678 nm and emission maximum at 699 nm, HiLyte Fluor™ 680 dyes are spectrally similar to Cy5.5™ dyes. Fluorescence emission of HiLyte Fluor™ 680 dyes is well separated from that of other commonly used red fluorophores, such as TAMRA, R-phycoerythrin and HiLyte Fluor™ 647 dyes, making it ideal for three and four-color labeling. Extinction coefficient is 190,000 M-1cm-1, with a quantum yield of 0.36.
Spectrally similar to Cy7™ dye, HiLyte Fluor™ 750 dye is the longest-wavelength HiLyte Fluor™ dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores, including HiLyte Fluor™ 647, HiLyte Fluor™ 680 or allophycocyanin (APC), facilitating multicolor analysis. Extinction coefficient is 275,000 M<1cm1, with a quantum yield of 0.7. With a peak excitation at ~753 nm, conjugates of HiLyte Fluor™ 750 dyes can be excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range. Its near infrared spectra render the HiLyte Fluor™ 750 dye the ideal candidate for in vitro and in vivo tissue imaging.

For more information, please visit anaspec.com or Eurogentec.com/.

 
 
Your Banner Ad Here instead - Showing along with ALL Articles covering Pharma/BioTech/Nutrition Announcements

Replace these Affiliate Programs at ANYTIME! Your banner here within the next hour. Learn How!


 

Written by / Agency / Source: AnaSpec, Inc.

 
 

Availability: All Regions (Including Int'l)

 

Traffic Booster: [/] Quick Newswire Today Visibility Checker

 

Distribution / Indexing: [+]

 
 
# # #
 

 
  Your Banner Ad showing on ALL
Pharma/BioTech/Nutrition articles,
CATCH Visitors via Your Competitors Announcements!


AnaSpec Highlights its Proprietary HiLyte Fluor™ Dye Collection

Company website links NOT available to basic submissions
It is OK to republish and/or LINK any newswire for any legitimate media purpose as long as you name Newswire Today and LINK as the source.
 
  Is this your article?
Activate ALL web links and social stream by Upgrading to Press Release PREMIUM Plan Now!

|
Publisher Contact: Tiffany Kao - AnaSpec.com 
510-791-9560
 
Newswire Today - PRZOOM / PRTODAY disclaims any content contained in this article. If you need/wish to contact the company who published the current release, you will need to contact them - NOT us. Issuers of articles are solely responsible for the accuracy of their content. Our complete disclaimer appears here.
IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

Pharma/BioTech/Nutrition via RSSAdd NewswireToday - PRZOOM Headline News to FeedBurner
Find who RetweetFollow @NewswireTODAY



Are you the owner of this article?, Turn it PREMIUM with your LOGO instead - and make it 3rd party Ads-Free! within the next hour!


Read Latest Articles From AnaSpec, Inc. / Company Profile


Read Pharma/BioTech/Nutrition Most Recent Related Newswires:

MDxHealth Signs Agreement with Lab21 to Make SelectMDx Liquid Biopsy Test Available in the United Kingdom
Greenphire Drives Global Site Adoption of Clinical Trial Payment Solutions
SelectMDx Chosen by Michigan Medicine as Pre-biopsy Diagnostic Tool in Groundbreaking US Prostate Cancer Risk Clinic
BTG Announces CE Mark Certification for DC Bead LUMI™ - The First Commercially Available Radiopaque Drug-Eluting Bead
Sartorius to Acquire Real-time Live-cell Analysis Pioneer and Leader Essen BioScience
Pyxis™ ES System to Help U.S. Department of Defense Advance Medication Safety and Patient Care
BTG Receives U.S. FDA 510(k) Clearance for EKOS® Control Unit 4.0
MDxHealth Announces Agreement with Istituto Diagnostico Varelli for Distribution of SelectMDx Throughout Central-South Italy
BD Veritor™ System Meets FDA’s New Performance Requirements for Rapid Influenza Antigen Detection Tests
Sub-Sahara Africa - Increase in Western Lifestyles Leads to Dire Need for Chronic Prescription Drugs Finds Frost & Sullivan
BD Initiates New Industry Alliance to Combat Rising Opioid Crisis
BTG and Society of Interventional Oncology to Explore Role of Minimally Invasive Therapies in Immuno-oncology
Ipsen to Acquire a Portfolio of Select Consumer Healthcare Products from Sanofi
BD Announces Early Access Program for BD Resolve™ Single-Cell Analysis Platform
Precision Medicine Unlocks the Future of Targeted Therapy in the Global Healthcare Industry Finds Frost & Sullivan

Boost Your Social Network
& Crowdfunding Campaigns


LIFETIME SOCIAL MEDIA WALL
NewswireToday Celebrates 10 Years in Business


PREMIUM Members


Visit  BizJobs.com

Visit  NAKIVO, Inc.





 
  ©2017 Newswire Today — Limelon Advertising, Co.
Home | About | Advertise/Pricing | Contact | Investors | Privacy/TOS | Sitemap | FRANCAIS
newswire, PR free press releases distribution service magazines engine news alert newsroom press room breaking news public relations articles company news alerts newswiredistribution ezine bizentrepreneur biznewstoday digital business report market search pr firms agencies reports distri-bution today investor relation successful internet entrepreneurs newswire distribution prtoday.com freenewswiredistribution asianewstoday bizwiretoday USA pr UK today - NOT affiliated with PRNewswire as we declined their partnership offer in 2013
 
PRTODAY & NewswireTODAY are NOT affiliated with USA TODAY (usatoday.com)