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Fremont, CA, United States, 2012/01/13 - Calcein AM (Calcein acetoxymethyl ester), a non-fluorescent, cell permeable compound, converts to the strongly green fluorescent calcein when hydrolyzed by intracellular esterases in live cells.1-2.
It is widely used for determining cell viability since the fluorescence intensity of calcein is proportional to the amount of live cells. Calcein is a photostable reagent and is well retained in the cytoplasm of live cells. Calcein is not influenced by intracellular pH. Its bright green fluorescence can be monitored at Ex/Em=494nm /520 nm.
Amongst dye reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is the most suitable indicator for staining viable cells due to its low cytotoxicity property. Calcein AM does not affect cell proliferation or viability.2-3 Calcein AM is used in cell invasion, adhesion, migration and other cell-based assays.
AnaSpec is pleased to provide Calcein AM at two purity grades, >90% or >95%, as determined by HPLC. AnaSpec also offers the SensoLyte® Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect viable cells. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format. For bulk quantity purchase, please email service[.]anaspec.com.
AnaSpec’s Calcein AM, a green fluorescent cell viability indicator, has excellent quality, and is cost-effective.
1. Braut-Boucher, F. et al. J. Immunol. Methods 178, 41 (1995).
2. Akeson, AL. et al. J. Immunol. Methods 163, 181 (1993).
3. Weston, SA. et al. J. Immunol. Methods 133, 87 (1990).
AnaSpec, Eurogentec Group
AnaSpec, Eurogentec Group (anaspec.com / Eurogentec.com) is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.